Genome management and mismanagement--cell-level opportunities and challenges of whole-genome duplication.

Gene Family: Structure, Organization and Evolution

  Gene families are considered as groups of homologous genes which they share very similar sequences and they may have identical functions. Members of gene families may be found in tandem repeats or interspersed through the genome. These sequences are copies of the ancestral genes which have underwent changes. The multiple copies of each gene in a family were constructed based on gene duplicati...

I-37: Establishing High Resolution Genomic Profiles of Single Cells Using Microarray and Next-Generation Sequencing Technologies

The nature and pace of genome mutation is largely unknown. Standard methods to investigate DNA-mutation rely on arraying or sequencing DNA from a population of cells, hence the genetic composition of individual cells is lost and de novo mutation in cell(s) is concealed within the bulk signal. We developed methods based on (SNP-) arraying and next-generation sequencing of single-cell whole-genom...

Whole Genome Sequencing: An Efficient Approach in Food Safety Management System

Post-translational changes of histones, methylation level, and ERβ protein level in the cumulus cell genome of infertile women with endometriosis

Background: Endometriosis (which affects up to 50% of infertile women) is one of the major causes impacting female infertility. Endometriosis, defined as the presence of endometrial glands and stroma outside the uterine tissue, causes a wide range of functional disorders in the process of follicular development and changes in the follicular milieu, resulting in the formation of an incompetent o...

Advancing Chimeric Antigen Receptor-Engineered T-Cell Immunotherapy Using Genome Editing Technologies: Challenges and Future Prospects

Chimeric antigen receptor engineered-T (CAR-T) cells also named as living drugs, have been recently known as a breakthrough technology and were applied as an adoptive immunotherapy against different types of cancer. They also attracted widespread interest because of the success of B-cell malignancy therapy achieved by anti-CD19 CAR-T cells. Current genetic toolbox enabled the synthesis of CARs ...